Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistance.
نویسندگان
چکیده
Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.
منابع مشابه
Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58.
Agrobacterium tumefaciens C58 and its derivatives give rise to spontaneous mutants resistant to tetracycline at a high frequency. We observed that a mutation affecting a tRNA processing function significantly affected the emergence of such mutants, suggesting that C58 contained a positively acting gene conferring resistance to tetracycline. A cosmid clone conferring resistance to tetracycline i...
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عنوان ژورنال:
- Molecular plant-microbe interactions : MPMI
دوره 14 1 شماره
صفحات -
تاریخ انتشار 2001